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freezone 1 l benchtop lyophilizer  (Labconco)


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    Labconco freezone 1 l benchtop lyophilizer
    Freezone 1 L Benchtop Lyophilizer, supplied by Labconco, used in various techniques. Bioz Stars score: 99/100, based on 12594 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/freezone+1+l+benchtop+lyophilizer/FreeZone+Freeze+Dryer/pmc12649050-65-28-33
    Average 99 stars, based on 12594 article reviews
    freezone 1 l benchtop lyophilizer - by Bioz Stars, 2026-09
    99/100 stars

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    Concentration Assay:

    Article Title: Mesenchymal stem/stromal cells overexpressing leukemia inhibitory factor (LIF) promote arteriogenesis and functional recovery in a mouse model of critical hindlimb ischemia
    Article Snippet: To obtain conditioned media, MSC and MSC_LIF cells were cultivated in 75 cm 2 culture flasks with 10 ml of EBM TM -2 medium (Lonza, Basel, Switzerland) without the addition of factors and supplements for 72 h. The culture supernatants were then collected in tubes and centrifuged at 3,000 rpm for 10 min to remove cellular debris. .. For concentration, 2 ml aliquots of the conditioned medium were placed in glass vials, frozen in a freezer at -80 °C for 1 h and placed in a FreeZone 1 L Benchtop lyophilizer (Labconco, Missouri, USA) at -50 °C and a pressure of 0.050 mbar for 24 h. Total protein dosage was determined by Pierce BCA Protein Assay (Thermo ScientificTM) and the lyophilisates were reconstituted in 1x PBS at a concentration of 200 μg/ml of total proteins. .. The determination of angiogenic proteins present in the conditioned medium of MSC and MSC_LIF was performed from immunoassay using Proteome Profiler Human Angiogenesis Array Kit (R&D Systems), following the manufacturer’s specifications.

    Bicinchoninic Acid Protein Assay:

    Article Title: Mesenchymal stem/stromal cells overexpressing leukemia inhibitory factor (LIF) promote arteriogenesis and functional recovery in a mouse model of critical hindlimb ischemia
    Article Snippet: To obtain conditioned media, MSC and MSC_LIF cells were cultivated in 75 cm 2 culture flasks with 10 ml of EBM TM -2 medium (Lonza, Basel, Switzerland) without the addition of factors and supplements for 72 h. The culture supernatants were then collected in tubes and centrifuged at 3,000 rpm for 10 min to remove cellular debris. .. For concentration, 2 ml aliquots of the conditioned medium were placed in glass vials, frozen in a freezer at -80 °C for 1 h and placed in a FreeZone 1 L Benchtop lyophilizer (Labconco, Missouri, USA) at -50 °C and a pressure of 0.050 mbar for 24 h. Total protein dosage was determined by Pierce BCA Protein Assay (Thermo ScientificTM) and the lyophilisates were reconstituted in 1x PBS at a concentration of 200 μg/ml of total proteins. .. The determination of angiogenic proteins present in the conditioned medium of MSC and MSC_LIF was performed from immunoassay using Proteome Profiler Human Angiogenesis Array Kit (R&D Systems), following the manufacturer’s specifications.



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